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Scientific Experience

Research Technician III

Duke University - Department of Ophthalmology

June 2017 - Present

I am currently a research technician at Duke University working on glaucoma research. I work on several projects within Dr. Paloma Liton's lab, all attempting to understand how autophagy plays a role in the functional failure of the trabecular meshwork and how it relates to glaucoma. 

Skills Gained:

  • Tissue culture

  • Western blotting

  • RNA isolation

  • qPCR

  • Genotyping

  • Animal handling

  • Immunohistochemistry

  • Immunocytochemistry

  • Confocal Imaging

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Publications:
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"Transcriptome analysis reveals autophagy as regulator of TGFβ/Smad-induced fibrogenesis in trabecular meshwork cells"

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"The autophagic protein LC3 translocates to the nucleus and localizes in the nucleolus associated to NUFIP1 in response to cyclic mechanical stress"

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"Autophagy in the Aging and Experimental Ocular Hypertensive Mouse Model"

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"Cathepsin B Localizes in the Caveolae and Participates in the Proteolytic Cascade in Trabecular Meshwork Cells. Potential New Drug Target for the Treatment of Glaucoma"

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"Primary cilia and the reciprocal activation of AKT and SMAD2/3 regulate stretch-induced autophagy in trabecular meshwork cells"

Biochemical Genetics Technologist

Laboratory Corporation of America - Center for Molecular Biology and Pathology

August 2015 - June 2017

As a laboratory technologist at LabCorp, I gained experience in a CAP, CLIA and ISO inspected facility while performing maternal serum screening. Additionally, I was the 5S and quality assurance representatives for the department. 

Skills Gained:

  • Gel electrophoresis

  • Chromatogram analysis

  • Dried blot spot preparation

  • Auditing experience

  • Leadership

  • Verbal communication

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Scientific Coursework

Undergraduate Coursework:

Microbiology: Fundamentals of Microbiology, Microbial Genetics, Bacterial Physiology, Bacterial Diversity, Marine Microbiology, Pathogenic Bacteriology, Food Microbiology

Chemistry: General Chemistry I & II, Organic Chemistry, Analytical Chemistry, Biochemistry I & II

Biology: General Biology, Human Anatomy and Physiology I & II

Other: Physics I & II

Graduate Coursework:

Microbiology: Microbial Metabolic Regulation, Industry Case Studies, Microbial Biotechnology

Biotechnology: Animal Cell Culture, Tissue Engineering

Biomanufacturing: Global Regulatory Affairs for Medical Products, Biological Processing Science

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